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Sinusoidal endothelial cells Generally the gradients used for the NPC isolation are similar to those described in Sections 1 and 2, Further purification is then achieved by suspension of the cells in DMEM supplemented with 10% fetal bovine serum and 1% L-glutamine (plus the usual antibiotics) and adherence to culture dishes coated with human fibronectin (1 mg/cm2) [9], by elutriation [3,4], using magnetic beads [13] or by flow cytometry [14]