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Jeff Rose | College of Health

RNA isolation and RT–qPCR Total RNAs from HeLa and HT-29 cells with or without the respective treatment were extracted using the RNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions From the cDNA generated by the Iscript Reverse Transcription Supermix (Bio-Rad), RT–qPCR was performed using Itaq Universal SYBR (Bio-Rad), with the primers listed in Supplementary Table 1 and the following conditions: initial polymerase activation step of 30 s at 95 °C, followed by 35 amplification cycles of 5 s at 95 °C and 30 s at 64 °C

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Color:215P - Ivory

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