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The supernatant after centrifugation was loaded onto a Ni-NTA column in the buffer containing 20 mM MOPS, pH 7.4, 100 mM NaCl, and 0.1% (w/v) digitonin, and the eluted fraction including the protein of interest was loaded onto a Superdex 200 (GE Healthcare) column equilibrated in a buffer containing 20 mM MOPS, pH 7.4, 100 mM NaCl and 0.1% (w/v) digitonin