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In these experiments, where the functional response measured was again MOPr activation of a G protein-coupled inwardly rectifying K + current (Bailey et al ., 2003), DAMGO induced rapid PKC-independent desensitization of the MOPr response, whereas morphine could only do so when PKC activity in the neurons was elevated, either by activating G q -coupled muscarinic acetylcholine receptors on the neurons or by addition of a phorbol ester to directly activate PKC (Bailey et al ., 2004, 2006)