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Whole cell and mitochondrial proteomics After the indicated culture conditions, cell pellets were processed as per immunoblot protocol and lysed with lysis buffer (50 mM Tris-Cl pH 7.5, 150 mM NaCl, 1 mM EDTA, 2% Triton X-100) supplemented with protease inhibitors and phosphatase inhibitors (Roche)

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Artificial intelligence-driven integration of genetic, metabolic, and lifestyle data could facilitate dynamic, patient-specific regimens, moving beyond empirical dosing toward adaptive precision care