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In summary, 1 mL of a solution comprising 80% v / v Type I rat tail collagen (2 mg/mL in 0.6% acetic acid, Thermo Fischer, Milan, Italy), 10% v / v Minimum Essential Medium (MEM, Merck Life Science, Milan, Italy), 5.8% v / v neutralising solution (Biosystems, Monza, Italy), and 4.2% Schwann cell suspension (4 × 10 6 RSC96 cells per 1 mL gel) was placed inside a rectangular scaffold measuring 16.4 mm by 6.5 mm by 5 mm