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The optimized HBA module reaction system contained 0.1 μM HemB, 1 μM HemC, 1 μM HemD, 10 μM CobA, 5 μM MetK, 5 μM PpK, 10 μM MtnN, 2 mM AMP, 1 mM SMPP, 5 mM ALA, 1 mM l-Met, 0.236 mM NADH, 0.236 mM NADPH, 5 mM MgCl 2 , 10 mM KCl, 5 mM NaCl, HBA crude cell extract of 155.8 mg/ml wet cell weight HBA cell culture fluid per volume reactant (detail information was illustrated in Supplementary Fig