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For Fluorescent Nissl Staining, brain sections were rinsed with 0.1 M Phosphate buffer (PB) for 5 min, and then incubated in a 0.1 M PB solution containing NeuroTrace™ 530/615 red fluorescent Nissl stain (1:2000), DAPI (1:100), 1% Triton X-100 in 0.1 M PB [rabbit anti-Nissl monoclonal antibody (1:1500)] overnight at 4°C and protected from light

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Its most clinically noticeable early effect for most patients is improved sleep quality and morning energy, driven by pineal gland stimulation and melatonin normalization, typically visible within weeks two to three